1WF3

Crystal structure of GTP-binding protein TT1341 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 41
Unit cell lengths	68.634, 68.634, 86.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.890 - 1.880
R-factor	0.2
Rwork	0.200
R-free	0.22700
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.900	1.950
High resolution limit [Å]	1.880	1.880
Number of reflections	32500
Completeness [%]	99.5	100
Redundancy	7.53932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1M Bicin, 5% v/v Glycerol, 10% w/v PEG8000, 4% v/v Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1M Bicin, 5% v/v Glycerol, 10% w/v PEG8000, 4% v/v Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K