1WF3
Crystal structure of GTP-binding protein TT1341 from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 |
Unit cell lengths | 68.634, 68.634, 86.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.890 - 1.880 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.22700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.900 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 32500 | |
Completeness [%] | 99.5 | 100 |
Redundancy | 7.53932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Bicin, 5% v/v Glycerol, 10% w/v PEG8000, 4% v/v Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Bicin, 5% v/v Glycerol, 10% w/v PEG8000, 4% v/v Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |