1WEK

Crystal structure of the conserved hypothetical protein TT1465 from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-03
Detector	MARRESEARCH
Wavelength(s)	0.979099, 0.979377, 0.974000
Spacegroup name	P 21 21 21
Unit cell lengths	65.650, 83.798, 265.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.940 - 2.200
R-factor	0.198
Rwork	0.198
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	70481
<I/σ(I)>	16.5034	3.46074
Completeness [%]	92.5	79.1
Redundancy	7.05944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP