1WEJ

IGG1 FAB FRAGMENT (OF E8 ANTIBODY) COMPLEXED WITH HORSE CYTOCHROME C AT 1.8 A RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1995-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.580, 72.540, 94.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.199 *
Rwork	0.200
R-free	0.25500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2.26 A RESOLUTION FABE8 X-RAY STRUCTURE (FREE FAB E8) (MYLVAGANAM ET AL. 1998 JMB 281 301-32; PDB ENTRY 1QBL) AND HORSE CYTOCHROME C 1.9 A X-RAY STRUCTURE (BUSHNELL ET. AL. 1990; PDB ENTRY 1HRC).
RMSD bond length	0.010 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.084 *
Number of reflections	48334 *
<I/σ(I)>	36.6	12
Completeness [%]	94.1	99.5
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.4		50MM ZINC ACETATE BUFFER, PH 6.4, 25% (W/V) PEG 4000.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	25 (%(w/v))
2	1	reservoir	zinc acetate	50 (mM)