1WDX
Yeast BBC1 SH3 domain, triclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.730, 44.770, 51.120 |
| Unit cell angles | 89.83, 83.37, 85.41 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.26594 |
| Rwork | 0.263 |
| R-free | 0.32595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | atomic-resolution structure of yeast bbc1 SH3 domain |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 11615 | |
| <I/σ(I)> | 3.9 | 2.3 |
| Completeness [%] | 90.8 | 92.5 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
