1WDV

Crystal structure of hypothetical protein APE2540

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-27
Detector	RIGAKU JUPITER
Wavelength(s)	0.964, 0.9792, 0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	47.435, 58.917, 53.603
Unit cell angles	90.00, 106.80, 90.00

Refinement procedure

Resolution	29.980 - 1.700
R-factor	0.169
Rwork	0.169
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	31231
<I/σ(I)>	20.9	13.5
Completeness [%]	99.9	100
Redundancy	5.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6	293	16.5% PEG20000, 0.1M HEPES pH 7.6, micro batch, temperature 293K