1WDS
The role of an inner loop in the catalytic mechanism of soybean beta-amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-03 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.615, 84.615, 143.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.640 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.20000 |
| Structure solution method | rigid body refinement |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | SCALE |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.432 |
| High resolution limit [Å] | 1.380 | 1.383 |
| Number of reflections | 114150 | |
| Completeness [%] | 94.0 | 73.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
