1WD9
Calcium bound form of human peptidylarginine deiminase type4 (PAD4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.389, 60.094, 115.339 |
| Unit cell angles | 90.00, 124.37, 90.00 |
Refinement procedure
| Resolution | 22.800 - 2.600 |
| R-factor | 0.22828 |
| Rwork | 0.224 |
| R-free | 0.26885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.662 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.361 |
| Number of reflections | 24864 | |
| <I/σ(I)> | 25.1 | 8.7 |
| Completeness [%] | 96.2 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEGMME2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
