1WD8
Calcium free form of human peptidylarginine deiminase type4 (PAD4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-18 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.612, 60.437, 113.488 |
| Unit cell angles | 90.00, 123.61, 90.00 |
Refinement procedure
| Resolution | 33.440 - 2.800 |
| R-factor | 0.23428 |
| Rwork | 0.231 |
| R-free | 0.26444 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.894 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.046 | 0.261 |
| Number of reflections | 20164 | |
| <I/σ(I)> | 12.7 | 3.1 |
| Completeness [%] | 97.6 | 98.3 |
| Redundancy | 1.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEGMME2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
