1WD3

Crystal structure of arabinofuranosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	39.455, 96.894, 145.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.460 - 1.750
R-factor	0.191
Rwork	0.191
R-free	0.20900
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	57830
Completeness [%]	99.0	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	PEG6000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K