1WD0
Crystal structures of the hyperthermophilic chromosomal protein Sac7d in complex with DNA decamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.970, 50.911, 51.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb 1azp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.930 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | 0.490 |
| Number of reflections | 8591 | |
| <I/σ(I)> | 43 | 2.3 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 6.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Tris-HCl, PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | PEG400 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Tris-HCl | ||
| 5 | 1 | 2 | PEG400 | ||
| 6 | 1 | 2 | H2O |
