1WCK
Crystal structure of the C-terminal domain of BclA, the major antigen of the exosporium of the Bacillus anthracis spore.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 68.558, 68.558, 163.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.650 - 1.360 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.450 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 48836 | |
| <I/σ(I)> | 35.4 | 4.24 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 18.5 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 20% PEG 4000, 20% ISOPROPANOL, 0.1M NA-CACODYLATE PH = 6.5, 10 MG/ML PROTEIN, VAPOUR DIFFUSION TECHNIQUE |
