1WCG
Aphid myrosinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.853, 67.365, 97.301 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 19.650 - 1.100 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.14600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.172 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.650 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 384672 | |
| <I/σ(I)> | 3.7 | 3.6 |
| Completeness [%] | 87.0 | 42.4 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | APHID MYROSINASE IN 20 MM TRIS PH 8.0, 150 MM NACL, 10 MM MGCL2 AND 5MM DDT AT 10 MG/ML. CRYSTALS WERE GROWN IN HANGING DROPS WITH PRECIPITANT 0.2M SODIUM FORMATE, 20% PEG3350 MIXED TO THE PROTEIN AT A RATIO OF 1:1. |
