1WC7
FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-04 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.476, 81.691, 79.330 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.330 |
| R-factor | 0.2222 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAB 15A9 IN COMPLEX WITH PPL-L-LYSINE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.700 |
| Number of reflections | 34338 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.31 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 30% PEG 3350, 200MM SODIUM REMARK 280 IODIDE, 50MM SODIUM ACETATE, PH 6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |
