1WC2
Beta-1,4-D-endoglucanase Cel45A from blue mussel Mytilus edulis at 1.2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-15 |
| Detector | CUSTOM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.470, 52.407, 80.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.200 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.16200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.453 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.079 | 0.171 |
| Number of reflections | 60648 | |
| <I/σ(I)> | 26.7 | 7.7 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6.4 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | EQUILIBRATION AGAINST 0.6 M NAAC PH 5.5 AND 0.1-0.5 M NACL |
