1WC0

Soluble adenylyl cyclase CyaC from S. platensis in complex with alpha, beta-methylene-ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	53.672, 71.539, 99.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.880 - 2.400
R-factor	0.219
Rwork	0.219
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Rmerge	0.080
Number of reflections	15574
<I/σ(I)>	7
Completeness [%]	99.2
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1