1WBP
SRPK1 bound to 9mer docking motif peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2003-11-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.680, 78.680, 310.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.260 - 2.400 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wak |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 * | 0.565 * |
| Total number of observations | 255743 * | |
| Number of reflections | 22765 | |
| <I/σ(I)> | 8.1 | 2.9 |
| Completeness [%] | 97.8 | 84 |
| Redundancy | 11 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 18 * | 100MM SODIUM CITRATE PH5.6, 200MM AMMONIUM ACETATE, 15%PEG3350, 5MM PEPTIDE, 5MM ADP, 10MM MGCL2, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 6 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 15 (%) |
