1WBL

WINGED BEAN LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-10
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	157.585, 91.908, 73.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.500
R-factor	0.187
Rwork	0.187
R-free	0.25900
Starting model (for MR)	ERYTHRINA CORALLODENDRON LECTIN (FOR MOLECULAR REPLACEMENT)
RMSD bond length	0.010
RMSD bond angle	27.300 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.630	2.650
High resolution limit [Å]	2.430	2.490
Rmerge	0.064	0.215
Total number of observations	50842 *
Number of reflections	29837
Completeness [%]	77.3	39.8 *
Redundancy	2.24	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	20 *	CRYSTALS WERE GROWN BY SITTING DROP METHOD IN WHICH 25 MICROLITERS OF AN 80 MG/ML PROTEIN SOLUTION IN 0.02M PHOSPHATE BUFFER AT PH 7.0, CONTAINING 0.15M SODIUM CHLORIDE, 0.025 (W/V) SODIUM NITRITE, 20 TIMES MOLAR EXCESS OF METHYL-ALPHA-D-GALACTOSE AND 6 TO 7.5% (W/V) PEG8000 WAS EQUILIBRATED AT 20 DEGREE CENTIGRADE WITH A RESERVOIR SOLUTION OF 40% PEG8000 IN THE SAME BUFFER., vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	80 (mg/ml)
10	1	reservoir		0.025 (%(w/v))
11	1	reservoir	methyl-alpha-D-galactopyranoside	20 (M)
2	1	drop	phosphate	0.02 (M)
3	1	drop		0.15 (M)
4	1	drop		0.025 (%(w/v))
5	1	drop	methyl-alpha-D-galactopyranoside	20 (M)
6	1	drop	PEG8000	6-7.5 (%(w/v))
7	1	reservoir	PEG8000	40 (%)
8	1	reservoir	phosphate	0.02 (M)
9	1	reservoir		0.15 (M)