1WBF
WINGED BEAN LECTIN, SACCHARIDE FREE FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.159, 75.161, 95.772 |
| Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.197 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wbl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.600 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.359 |
| Total number of observations | 38947 * | |
| Number of reflections | 26671 | |
| <I/σ(I)> | 12.5 | 2.8 |
| Completeness [%] | 88.7 | 80.6 |
| Redundancy | 1.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 20 * | CRYSTALS WERE GROWN BY BATCH METHOD IN WHICH 25 MICROLITERS OF AN 80 MG/ML PROTEIN SOLUTION IN 0.02M PHOSPHATE BUFFER AT PH 7.0, CONTAINING 0.15M SODIUM CHLORIDE, 0.025 (W/V) SODIUM AZIDE, WAS MIXED WITH 45% MPD IN THE SAME BUFFER AT 20 DEGREES CENTIGRADE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 1 | phosphate | 0.02 (M) | |
| 3 | 1 | 1 | 0.15 (M) | ||
| 4 | 1 | 1 | 0.025 (%(w/v)) | ||
| 5 | 1 | 1 | PEG4000 | 6-7.5 (%(w/v)) | |
| 6 | 1 | 1 | sugar | 20 times molar excess | |
| 7 | 1 | reservoir | phosphate | 0.02 (M) | |
| 8 | 1 | reservoir | PEG4000 | 40 (%) |
