1WAJ

DNA POLYMERASE FROM BACTERIOPHAGE RB69

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-09
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	81.410, 117.600, 199.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.800
R-factor	0.21
Rwork	0.210
R-free	0.28000
Structure solution method	MIR
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.800
Rmerge	0.089
Number of reflections	42608
Completeness [%]	89.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6	4 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	sodium citrate	0.05 (M)
3	1	drop	sodium potassium tartrate	0.025 (M)
4	1	drop	ammonium sulfate	0.5 (M)
5	1	reservoir	sodium citrate	0.1 (M)
6	1	reservoir	sodium potassium tartrate	0.5 (M)
7	1	reservoir	ammonium sulfate	1.0 (M)