1WAF
DNA POLYMERASE FROM BACTERIOPHAGE RB69
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | MAC Science DIP-2000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.320, 196.860, 118.310 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.27400 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.145 |
| Number of reflections | 60256 |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 4 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.05 (M) | |
| 3 | 1 | reservoir | sodium potassium tartrate | 0.025 (M) | |
| 4 | 1 | reservoir | 0.25 (M) | ||
| 5 | 1 | reservoir | ammonium sulfate | 0.25 (M) |
