1W9X
Bacillus halmapalus alpha amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.180, 75.847, 155.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3x |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 30048 | |
| <I/σ(I)> | 14 | 5 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30 MGML-1 IN 50 MM IMIDAZOLE, 5 MM CACL2, 25 MM ACARBOSE, PH 7.5 MIXED IN 1:1 RATIO WITH THE WELL SOLUTION CONSISTING OF 0.1 M IMIDAZOLE PH 7.5 100 MM MALTOSE, 5 MM CACL2, AND WITH 18% (W/V) MONOMETHYLETHER POLYETHYLENE GLYCOL 5K AS PRECIPITANT. |
