1W9K

Dictyostelium discoideum Myosin II motor domain S456E with bound MgADP-BeFx

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-30
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	104.704, 179.428, 54.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.050
R-factor	0.2
Rwork	0.198
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mma
RMSD bond length	0.011
RMSD bond angle	1.407
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.100
High resolution limit [Å]	2.000	2.050
Rmerge	0.060	0.260
Number of reflections	68455
<I/σ(I)>	24	4.3
Completeness [%]	96.0	96.2
Redundancy	6.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		10% PEG 8000, 50 MM HEPES PH 7, 100 MM NACL, 2 MM DTT, 2 MM NAN3 BY MICROSEEDING