1W9H

The Structure of a Piwi protein from Archaeoglobus fulgidus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	69.090, 137.863, 51.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.010 - 1.950
R-factor	0.194
Rwork	0.192
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.635
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SnB
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.050
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.160
Number of reflections	143262
<I/σ(I)>	18.6	6.4
Completeness [%]	98.6	92.8
Redundancy	4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		PROTEIN SOLUTION AT 10 MG/ML WAS MIXED WITH A SOLUTION CONTAINING 0.1 M SODIUM ACETATE PH 4.6, 0.1 M CADMIUM CHLORIDE, 30% PEG 400, 5 MM DTT. CRYSTALS COULD BE OBTAINED REPRODUCIBLY BY STREAK SEEDING IN TO THE CRYSTALLISATION SOLUTION AFTER A 3 HOUR EQUILIBRATION. CRYSTALS APPEARED AFTER 2-3 DAYS AND GREW AFTER 5 DAYS TO A FINAL SIZE OF 0.1 MM X 0.1 MM X 0.1 MM