1W9C

Proteolytic fragment of CRM1 spanning six C-terminal HEAT repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	118.870, 62.870, 114.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.229
Rwork	0.229
R-free	0.27700
Structure solution method	OTHER
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.120	0.680
Number of reflections	35395
<I/σ(I)>	9.3	1.6
Completeness [%]	90.9	73.3
Redundancy	3.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8.00