1W96
Crystal Structure of Biotin Carboxylase Domain of Acetyl-coenzyme A Carboxylase from Saccharomyces cerevisiae in Complex with Soraphen A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Collection date | 2004-03-30 |
| Wavelength(s) | 0.978994,0.979232, 0.970227 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.830, 96.520, 139.950 |
| Unit cell angles | 90.00, 96.82, 90.00 |
Refinement procedure
| Resolution | 29.200 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 150099 | |
| <I/σ(I)> | 19.1 | 3 |
| Completeness [%] | 96.3 | 88 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
