1W8X

Structural analysis of PRD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	278
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	903.000, 920.600, 926.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 4.200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	4.270
High resolution limit [Å]	4.200	4.200
Rmerge	0.330	0.650
Number of reflections	2383561
<I/σ(I)>	1.8	0.9
Completeness [%]	45.0	7
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		3.7 W/V PEG 8000, 400 MM NACL 100 MM POTASSIUM PHOSPHATE BUFFER PH 7.2