1W8M
Enzymatic and Structural Characterisation of Non Peptide Ligand Cyclophilin Complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-20 |
| Detector | MARRESEARCH 300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.307, 54.536, 71.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.040 | 0.050 |
| Number of reflections | 17641 | |
| <I/σ(I)> | 16 | 10 |
| Completeness [%] | 90.4 | 86.1 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS.HCL (PH 8.0), 22% (W/V) PEG 8000, 5% (V/V) DMSO, 0.02% NAN3. |
