1W8L
Enzymatic and structural characterization of non peptide ligand cyclophilin complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-10 |
| Detector | MARRESEARCH 300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.090, 54.310, 70.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.250 |
| Number of reflections | 13305 | |
| <I/σ(I)> | 16.5 | 2.5 |
| Completeness [%] | 98.7 | 90.7 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS.HCL (PH 8.0), 22% (W/V) PEG 8000, 5% (V/V) DMSO, 0.02% NAN3. |
