1W8K

Crystal structure of apical membrane antigen 1 from Plasmodium vivax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-14
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	150.030, 53.760, 60.290
Unit cell angles	90.00, 113.18, 90.00

Refinement procedure

Resolution	19.340 - 1.800
R-factor	0.222
Rwork	0.219
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.926
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.060	0.740
Number of reflections	152093
<I/σ(I)>	10.5	2
Completeness [%]	99.2	97.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		THE CRYSTALLISATION MIXTURE CONSISTED OF 10-12% (W/V) PEG3350, 100-200MM IMIDAZOLE, PH 7.0, 5-10% (V/V) ISOPROPANOL, AND 1% (V/V) DMF OR 3% (V/V) T-BUTANOL. THE PROTEIN SOLUTION WAS DILUTED BY 40 - 60% REMARK 280 WITH THE CRYSTALLISATION BUFFER, GIVING A FINAL REMARK 280 CONCENTRATION OF 4-6 MG. ML-1 IN SUSPENDED DROPS OF VOLUME REMARK 280 2 - 3 PERCENT.