1W8J
Crystal Structure Of Myosin V Motor Domain - Nucleotide-Free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 134.476, 162.308, 174.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.520 - 2.700 |
| R-factor | 0.2574 |
| Rwork | 0.255 |
| R-free | 0.30830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.353 |
| Number of reflections | 99891 | |
| <I/σ(I)> | 4.56 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 10% PEG 8000, 50 MM MES PH 6.3, 100 MM AMMONIUM SULPHATE, 2 |
