1W87
FERREDOXIN-NADP REDUCTASE (MUTATION: Y 303 W) COMPLEXED WITH NADP BY COCRYSTALLIZATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-07-07 |
| Detector | BRUKER-NONIUS |
| Spacegroup name | I 4 |
| Unit cell lengths | 221.270, 221.270, 37.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.990 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gjr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.990 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.010 | 0.410 |
| Number of reflections | 16780 | |
| <I/σ(I)> | 3.9 | 1.3 |
| Completeness [%] | 83.6 | 48.2 |
| Redundancy | 2.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.00 |
