1W7T
Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.308, 52.815, 141.944 |
| Unit cell angles | 90.00, 120.07, 90.00 |
Refinement procedure
| Resolution | 119.520 - 1.850 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.509 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.760 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.248 |
| Number of reflections | 85530 | |
| <I/σ(I)> | 5.5 | 2.9 |
| Completeness [%] | 97.6 | 86.6 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 277 | CRYSTALS WERE GROWN AT 4C FROM 50 MM MGCL2, 14-17 % PEG3350 AND 50-100 MM TRIS/CL PH 7.8 - 8.6. |
