1W7S
Wild-Type Aequorea victoria Green Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.968, 52.730, 141.628 |
| Unit cell angles | 90.00, 120.01, 90.00 |
Refinement procedure
| Resolution | 119.520 - 1.850 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.482 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.172 |
| Number of reflections | 85082 | |
| <I/σ(I)> | 6.7 | 4.2 |
| Completeness [%] | 97.5 | 86 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 277 | CRYSTALS WERE GROWN AT 4C FROM 50 MM MGCL2, 14-17 % PEG3350 AND 50-100 MM TRIS/CL PH 7.8 - 8.6. |
