1W7O

cytochrome c3 from Desulfomicrobium baculatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1992-10-15
Detector	MARRESEARCH
Spacegroup name	P 31
Unit cell lengths	43.000, 43.000, 64.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.410 - 1.810
R-factor	0.178
Rwork	0.176
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cy3
RMSD bond length	0.022
RMSD bond angle	1.948
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.500	1.900
High resolution limit [Å]	1.810	1.810
Rmerge	0.080	0.183
Number of reflections	11633
<I/σ(I)>	9.1	3
Completeness [%]	94.4	80
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		25% PEG 6K, 0.1 M TRIS-HCL, PH 7.5-8.0