1W7J
Crystal Structure Of Myosin V Motor With Essential Light Chain + ADP-BeFx - Near Rigor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.684, 95.988, 84.306 |
| Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe9 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.510 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.323 |
| Number of reflections | 86066 | |
| <I/σ(I)> | 14.9 | 3.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 12% PEG 8000, 50 MM MES PH 6.3, 2 MM DTT AND 2 MM NAN3 |
