1W7I
Crystal Structure Of Myosin V Motor Without nucleotide soaked in 10 mM MgADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.926, 99.253, 112.513 |
| Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
| Resolution | 111.800 - 3.000 |
| R-factor | 0.252 |
| Rwork | 0.248 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe9 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.800 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.081 | 0.284 |
| Number of reflections | 21972 | |
| <I/σ(I)> | 18.1 | 3.1 |
| Completeness [%] | 97.3 | 88.3 |
| Redundancy | 6.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 6% PEG 8000, 50 MM MOPS PH 6.5, 2 MM DTT AND 2 MM NAN3 |
