1W75
Native Orthorhombic form of Torpedo californica acetylcholinesterase (AChE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-01 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.502, 106.586, 150.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.562 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.270 |
| Number of reflections | 55093 | |
| <I/σ(I)> | 13 | 4 |
| Completeness [%] | 94.3 | 88.3 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN IN 100MM NACL, 0.01%NAN3, 1MM MES PH6.5, MIXED 1/1 WITH SOLUBILIZED LI5 (PW11O39RHCH2COOH), 3 MICROLITER OF THIS MIXTURE WAS MIXED WITH 3 MICROLITER OF 40% PEG200 IN 0.5M MES PH5.8, SUSPENDED OVER WELL WITH 36%PEG200 IN 0.5M MES, PH 5.8 |
