1W6X

SH3 domain of p40phox, component of the NADPH oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	35.160, 45.160, 35.060
Unit cell angles	90.00, 111.43, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.2476
Rwork	0.248
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.302
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.270
Number of reflections	6876
<I/σ(I)>	3.7	2.4
Completeness [%]	98.1	90
Redundancy	3.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		287	VAPOR DIFFUSION AT 14 DEGRE C, PROTEIN AT 14 MG/ML, 20 MM HEPES PH 7.5, 150 MM NACL MIXED WITH 40% PEG2K MME, 100 MM AS 200 MM NA AC PH 4.6.