1W5K
AN ANTI-PARALLEL FOUR HELIX BUNDLE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Collection date | 2003-01-09 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 33.886, 37.311, 104.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.410 - 1.920 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.30700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.310 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.050 | 0.250 |
| Number of reflections | 19519 | |
| <I/σ(I)> | 9.4 | 2.3 |
| Completeness [%] | 98.7 | 96.5 |
| Redundancy | 2.69 | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | HANGING DROP, 1UL OF 10MG/ML PEPTIDE IN WATER, 1UL 100MM CAPS, 30% PEG 400, PH 10.5, 1UL OF 50MM TCEP IN WATER |
