1W5B

FtsZ dimer, GTP soak (M. jannaschii)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	70.303, 55.203, 88.129
Unit cell angles	90.00, 90.97, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.209
Rwork	0.209
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fsz
RMSD bond length	0.006
RMSD bond angle	1.195
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.360
Number of reflections	32449
<I/σ(I)>	12.7	4.2
Completeness [%]	98.2	98.2
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1