1W4V
structure of the oxidised form of human thioredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.060, 49.140, 78.710 |
| Unit cell angles | 87.75, 82.77, 79.20 |
Refinement procedure
| Resolution | 19.320 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uvz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.947 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.270 |
| Number of reflections | 65984 | |
| <I/σ(I)> | 11.67 | 3.4 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 2.22 | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | PEG 3350 22% (W/V) NA ACETATE 0.1M (PH 4.6) AMMONIUM SULFATE 0.2M DTT 1MM. SOAKING IN A H2O2 SOLUTION 1MM |
