1W4O
Binding of Nonnatural 3'-Nucleotides to Ribonuclease A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-28 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.819, 32.556, 72.467 |
Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
Resolution | 22.180 - 1.600 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.510 |
Number of reflections | 30191 | |
<I/σ(I)> | 10.8 | 1.8 |
Completeness [%] | 89.9 | 88.5 |
Redundancy | 3.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 20MM SODIUM CITRATE BUFFER, PH5.5, PEG 4000 (25% W/V), pH 5.50 |