1W49
P4 protein from Bacteriophage PHI12 in complex with AMPcPP and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-21 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.700, 130.100, 159.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24590 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.250 |
| Number of reflections | 42708 | |
| <I/σ(I)> | 22.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM AMPCPP 10 MM MGCL, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROPS, 4C |
