1W42
T. celer L30e R92A variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.309, 53.780, 34.445 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 32.270 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.064 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.280 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 7440 | |
| <I/σ(I)> | 23.3 | 7.3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
