1W3S
The crystal structure of RecO from Deinococcus radiodurans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.429, 52.351, 101.085 |
| Unit cell angles | 90.00, 106.32, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.400 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 26328 | |
| <I/σ(I)> | 9.6 | 1.4 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M CALCIUM ACETATE, 8% (W/V) PEG 20000, 8% (W/V) PEG 550 MONOMETHYL ETHER (MME), 0.1 M TRIS/HCL PH 8.5 |
