1W3H
The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 120 |
Collection date | 2003-10-23 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 95.232, 95.232, 151.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 1.500 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clx |
RMSD bond length | 0.015 |
RMSD bond angle | 1.539 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.070 | 0.360 |
Number of reflections | 107241 | |
<I/σ(I)> | 19.94 | 2.7 |
Completeness [%] | 95.3 | 87.2 |
Redundancy | 5.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |