1W3E
Ribosomal L30e of Thermococcus celer, P59A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.194, 53.802, 34.174 |
| Unit cell angles | 90.00, 109.36, 90.00 |
Refinement procedure
| Resolution | 32.270 - 1.770 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.467 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.930 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.040 | 0.080 |
| Number of reflections | 7622 | |
| <I/σ(I)> | 24.9 | 11.7 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG 6000 0.1M HEPES PH 7.0 20% PEG 400 |
