1W35

FERREDOXIN-NADP+ REDUCTASE (MUTATION: Y 303 W)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	CCD
Collection date	2004-07-21
Spacegroup name	P 65
Unit cell lengths	87.085, 87.085, 96.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.290 - 1.900
R-factor	0.171
Rwork	0.171
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gjr
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.285	1.970
High resolution limit [Å]	1.902	1.900
Rmerge	0.142	0.351
Number of reflections	32488
<I/σ(I)>	14	3
Completeness [%]	100.0	100
Redundancy	9.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		pH 5.00