1W34

FERREDOXIN-NADP REDUCTASE (MUTATION: Y 303 S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2004-06-14
Spacegroup name	P 65
Unit cell lengths	86.330, 86.330, 96.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.010 - 1.730
R-factor	0.185
Rwork	0.185
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gjr
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.010	1.820
High resolution limit [Å]	1.730	1.730
Rmerge	0.060	0.250
Number of reflections	42423
<I/σ(I)>	6.7	2.9
Completeness [%]	99.6	97.6
Redundancy	4.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.50