1W33
BbCRASP-1 from Borrelia Burgdorferi
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-10 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.934, 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.803, 89.803, 145.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.958 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HYSS |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.257 |
| Number of reflections | 15999 | |
| <I/σ(I)> | 9.1 | 3 |
| Completeness [%] | 95.0 | 97.6 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 25% PEG 600 200 MM IMIDAZOLE PH 6.0 |
